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X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue).
Following protocol only provides a guideline, and should be modified according to your specific needs.
1. Dissolve 20 mg X-Gluc cyclohexanamine in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix.
2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization.
3. Allow the plates to air dry and be used to inoculate the organisms.
4. Incubate the plates at 35°C and observe 16-24 h after inoculation.
| Molecular Weight | 521.79 |
| Formula | C20H26BrClN2O7 |
| CAS Number | 114162-64-0 |
| Solubility (25°C) | DMF ≥ 20 mg/mL |
| Storage | 4°C, protect from light, dry |
| Related Fluorescent Dye Products |
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| Indocyanine green
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| Fluo-4 AM
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All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
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