All AbMole products are for research use only, cannot be used for human consumption.
WZ4003 is a highly specific NUAK kinase inhibitor with IC50 of 20 nM and 100 nM for NUAK1 and NUAK2, respectively, without significant inhibition on 139 other kinases.
J Agric Food Chem. 2017 Mar 15;65(10):2089-2099.
10-Gingerol, a Phytochemical Derivative from “Tongling White Ginger”, Inhibits Cervical Cancer: Insights into the Molecular Mechanism and Inhibitory Targets
WZ4003 purchased from AbMole
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Source | APMIS.m (2017). Figure 7. WZ4003 (Abmole Bioscience Inc. Houston, TX, USA) |
| Method | Western blot | |
| Cell Lines | HeLa cells | |
| Concentrations | ||
| Incubation Time | ||
| Results | Particularly, pifithrin-μ and WZ4003 dramatically decreased 10-G-induced activation of the p53 and p-AMPK protein, respectively. |
| Cell Experiment | |
|---|---|
| Cell lines | U2OS cells, and MEFs |
| Preparation method | Following the manufacturer’s protocol ,to carry out cell proliferation assays colorimetrically in 96-well plates using the CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay kit . Initially, 2000 cells per well are seeded for U2OS cells and 3000 cells per well are seeded for MEFs. The proliferation assays are carried out over 5 days in the presence or absence of 10 μM WZ4003. |
| Concentrations | ~10 μM |
| Incubation time | 5 days |
| Animal Experiment | |
|---|---|
| Animal models | |
| Formulation | |
| Dosages | |
| Administration | |
| Molecular Weight | 496.99 |
| Formula | C25H29ClN6O3 |
| CAS Number | 1214265-58-3 |
| Solubility (25°C) | DMSO ≥ 10 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
[2] Huali Yang, et al. Optimization of WZ4003 as NUAK inhibitors against human colorectal cancer
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