All AbMole products are for research use only, cannot be used for human consumption.
Vacuolin-1 is a cell-permeable inhibitor of Ca2+ dependent fusion of lysosomes to the cell membrane. Vacuolin-1 blocks the Ca2+-dependent exocytosis of lysosomes and prevents the release of lysosomal content without affecting the process of resealing. vacuolin‐1 is a potent and selective PIKfyve inhibitor and inhibits late‐stage autophagy by impairing lysosomal maturation.
Vacuolin-1 (1 μM; Pretreatment 1 hour) blocks ionomycin-induced exocytosis of lysosomes but not of enlargeosomes. It results in vacuolation of lysosomes (A) and complete inhibition of the surface expression of Lamp-1. Vacuolin-1 (1 μM; 20-180 min) disrupts the segregation of inner and limiting membranes characteristic of endosomes and lysosomes. Ultrastructural analysis also shows that vacuolin-1 favours fusion of inner and limiting membranes. Vacuolin-1 (5 or 10 μM; 2 hours) blocks the Ca2+-dependent release of β-hexosaminidase from lysosomes. In HeLa cells, ionomycin results in the expected release of 18-20% of lysosomal β-hexosaminidase. But when cells pretreated with vacuolin-1, releases no more β-hexosaminidase compared with cells that are not exposed to ionomycin (4%).
| Cell Experiment | |
|---|---|
| Cell lines | RBL 2H3 cells and BMMCs |
| Preparation method | RBL-2H3 cells or BMMCs were treated with various concentrations of inhibitors for 30 min before adding vacuolin-1 (final concentration 10 µM). After further 3 h incubation with the inhibitor and vacuolin-1, cell viability was assessed by trypan blue staining. |
| Concentrations | 10 µM |
| Incubation time | 3 h |
| Animal Experiment | |
|---|---|
| Animal models | BALB/c mice |
| Formulation | DMSO and saline |
| Dosages | 15, 20, 30 mg/kg |
| Administration | Oral and I.P. |
| Molecular Weight | 577.42 |
| Formula | C26H24IN7O |
| CAS Number | 351986-85-1 |
| Form | Solid |
| Solubility (25°C) | DMSO 6 mg/mL |
| Storage | -20°C, sealed |
| Related Autophagy Products |
|---|
| Tacrolimus (FK506)
Tacrolimus (FK506, FR900506, Fujimycin, Prograf) is a potent, clinically-useful immunosuppressant in the same molecular class as cyclosporin A and rapamycin. *The compound is unstable in solutions, freshly prepared is recommended |
| Metformin hydrochloride
Metformin HCl is a biguanide inhibitor that reduces blood glucose mainly by inhibiting hepatic glycogen production (hepatic gluconeogenesis), while Metformin is a CD47 inhibitor in vitro. |
| Ginkgolide K
Ginkgolide K induces protective autophagy through the AMPK/mTOR/ULK1 signaling pathway. |
| Ivermectin (MK-933)
Ivermectin (MK-933) is a positive allosteric modulator of the α7 neuronal nicotinic acetylcholine receptor and the purinergic P2X4 receptor. Importin α5 inhibitor ivermectin effectively abrogates STAT1-importin α5 interactions. |
| Bergapten
Bergapten is a psoralen that can be photoactivated and is capable of crossing-linking DNA, covalently modifying proteins and lipids, and consequently inhibiting cell replication. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2026 AbMole BioScience. All Rights Reserved.
