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Uricase catalyses the rapid oxidation of uric acid into allantoic acid, it is extracted from fermentation broths of Aspergillus niger, Aspergillus flavus, and Candida parapsilosis. It is employed in biochemical research and for the determination of uric acid in serum and urine.
Assay Definition: The amount of enzyme required to oxidise 1 μmol of uric acid per minute at pH 8.5 and 25 °C.
Isoelectric Point (PI): 5.4
pH Stability: 7.0–11.0
Optimal pH: 8.5
Thermal Stability: <50°C (pH 8.5, 10 min)
Optimal Temperature: 45°C
Method of Use: For activity measurement, dissolve lyophilised powder in 50 mM borate buffer (pH 8.5) containing 0.001% Triton X-100 and 1 mM EDTA.
| CAS Number | 9002-12-4 |
| Form | Lyophilized powder |
| Solubility (25°C) | Soluble in buffer alkaline solution |
| Storage | -20°C |
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