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TWS119 is a glycogen synthase kinase-3β inhibitor with an IC50 of 30 nM. TWS119 induces neuronal differentiation in pluripotent murine embryonal carcinoma cells and embryonic stem cells (ESCs) at 400 nM.
Front Pharmacol. 2023 Jun 29;14:1181319.
Liang-Ge-San: a classic traditional Chinese medicine formula, attenuates acute inflammation via targeting GSK3β
TWS119 purchased from AbMole
Aquaculture Reports. 2023 Dec;101784.
Squalene activates Wnt/β-Catenin signaling pathway to mediate NF-κB pathway to regulate inflammatory response and disease resistance in hybrid grouper
TWS119 purchased from AbMole
Biochim Biophys Acta Mol Cell Res. 2019 Mar;1866(3):486-503.
Protease-activated receptor 2 induces migration and promotes Slug-mediated epithelial-mesenchymal transition in lung adenocarcinoma cells.
TWS119 purchased from AbMole
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Source | Molecular Cancer Research (2018). Figure 5. TWS119 (AbMole BioScience, Houston, TX, USA) |
| Method | inactivate glycogen synthase kinase 3β | |
| Cell Lines | CL1-5 cells | |
| Concentrations | 50 nM | |
| Incubation Time | 18 h | |
| Results | In the scrambled control cells, treatment of CL1-5 cells with TWS119 did not affect the basal expression of EMT markers, Slug, and the phosphorylation of Ser9 of GSK3β |
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Source | Exp Cell Res (2018). Figure 1. TWS119 |
| Method | CFSE proliferation assay | |
| Cell Lines | γδT cells | |
| Concentrations | 2 μM | |
| Incubation Time | 72 h | |
| Results | CFSE dilution assays showed that treatment of PBMCs with TWS119 (2 μM) could simulate γδT cells to divide robustly and with less cell apoptosis compared with the control group |
| Cell Experiment | |
|---|---|
| Cell lines | megakaryocytes (MKs) |
| Preparation method | To examine the inhibitory effects of p38MAPK, PI3K, and GSK-3β on MK differentiation and platelet production, hBMMNCs were cultured in MKLI media in the presence or absence (+/−) of each of the following inhibitors for 12 days: 5 µM SB202190 (AbMole, Houston, TX, USA) to inhibit p38MAPK; 10 µM LY294002 (AbMole, Houston, TX, USA) to inhibit PI3K; and 1 µM TWS119 (AbMole, Houston, TX, USA) to inhibit GSK-3β. To investigate the effect of GSK-3β inhibition on platelet production from mature MKs defined as CD41 (+)/CD42b (+)/propidium iodide (PI) (−), as described later (flow cytometry analysis in the Methods), mature MKs were then cultured in MKLI media with TWS119 (+/−) for 6 days. |
| Concentrations | 0, 0.17, 1, and 3 µM |
| Incubation time | 12 days |
| Animal Experiment | |
|---|---|
| Animal models | Permanent focal cerebral ischemia rats |
| Formulation | 1 % DMSO |
| Dosages | 30 mg/kg |
| Administration | intraperitoneal |
| Molecular Weight | 318.33 |
| Formula | C18H14N4O2 |
| CAS Number | 601514-19-6 |
| Solubility (25°C) | DMSO 38 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Related GSK-3 Products |
|---|
| CHIR-99021
CHIR-99021 (CT99021) is an aminopyrimidine derivative that inhibits GSK3α and GSK3β with IC50 values of 10 and 6.7 nM, respectively. |
| CHIR-99021 HCl
CHIR-99021 HCl (Laduviglusib; CT99021) is a hydrochloride of CHIR-99021, with IC50 of 10 nM and 6.7 nM for GSK-3α and GSK-3β, respectively. |
| SB 216763
SB 216763 is a potent and selective, cell permeable glycogen synthase kinase-3 (GSK-3) inhibitor with an IC50 value of 34 nM for GSK-3α. |
| AZD2858
AZD2858 is a potent, orally bioactive inhibitor of GSK-3 with an IC50 of 68 nM. |
| Indirubin
Indirubin is a potent cyclin-dependent kinases and GSK-3β inhibitor with IC50 of ~75 nM and 0.19 μM. |
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