Tunicamycin

Biological Activity

Tunicamycin inhibits GlcNAc phosphotransferase (GPT).Tunicamycin blocks the formation of N-glycosidic linkages by inhibiting the first step in glycoprotein synthesis.Tunicamycin can be used to induce autophagy.Tunicamycin induces apoptosis in association with caspase-3 activation, generation of reactive oxygen species (ROS), and downregulation of survivin expression.P38 MAPK (mitogen-activated protein kinase) and the generation of ROS signaling pathway play crucial roles in TN-induced apoptosis in U937 cells.TN modulates multiple components of the apoptotic response of human leukemia cells and raise the possibility of a novel therapeutic strategy for hematological malignancies.

Product Citations

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  Life Sci. 2025 May 19;375:123714.

  The role of LECT2 in kidney fibrosis progression and endoplasmic reticulum stress
  Tunicamycin purchased from AbMole

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  Int J Surg. 2024 Dec 20.

  Loss of RPN1 Promotes Antitumor Immunity via PD-L1 Checkpoint Blockade in Triple-negative Breast Cancer——Experimental Studies
  Tunicamycin purchased from AbMole

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  Ecotoxicol Environ Saf. 2024 Jul 13.

  ATF4-mediated different mode of interaction between autophagy and mTOR determines cell fate dependent on the level of ER stress induced by Cr(VI)
  Tunicamycin purchased from AbMole

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  Toxicology. 2024 Jul 19.

  HMGA2-mediated glutamine metabolism is required for Cd-induced cell growth and cell migration
  Tunicamycin purchased from AbMole

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  Toxicology. 2024 Oct 18.

  ATF4/PHGDH mediates the effects of ER stress on cadmium-induced autophagy and glycolysis
  Tunicamycin purchased from AbMole

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  Tissue Cell. 2024 Aug 23;91:102531.

  BRD4 absence inactivates endoplasmic reticulum stress to retard dehydroepiandrosterone-triggered ovarian granular cell apoptosis in polycystic ovary syndrome via GRP78
  Tunicamycin purchased from AbMole

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  Stem Cells Int. 2023 Jul 26;2023:4483776.

  Mesenchymal Stem Cells Inhibit Epithelial-to-Mesenchymal Transition by Modulating the IRE1α Branch of the Endoplasmic Reticulum Stress Response
  Tunicamycin purchased from AbMole

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  Tissue Cell. 2023 Jul 26;84:102183.

  Krüppel-like factor 15 counteracts endoplasmic reticulum stress and suppresses lung fibroblast proliferation and extracellular matrix accumulation
  Tunicamycin purchased from AbMole

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  J Ethnopharmacol. 2022 October 5;296:115519.

  Lycium barbarum L. and Salvia miltiorrhiza Bunge protect retinal pigment epithelial cells through endoplasmic reticulum stress
  Tunicamycin purchased from AbMole

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  Research Square. 2022 Apr 13.

  Mesenchymal stem cells alleviate epithelial-to-mesenchymal transition by modulating the IRE1α branch of the endoplasmic reticulum stress response
  Tunicamycin purchased from AbMole

Protocol (for reference only)

Cell Experiment
Cell lines	EA.hy926-L2-OE and EA.hy926-NC cells
Preparation method	EA.hy926-L2-OE and EA.hy926-NC cells were cultured in six-well plates until they reached 70 % confluence. To induce ERS, the cells were treated with 1 μg/mL of tunicamycin (M4798, AbMole) for 24 h, with DMSO serving as the control.
Concentrations	1 μg/mL
Incubation time	24 h

Animal Experiment
Animal models	C57BL/6 and Lect2-KO mice
Formulation	DMSO
Dosages	1 mg/kg
Administration	Intraperitoneal injection

Chemical Information

Molecular Weight	816.89
Formula	C37H60N4O16
CAS Number	11089-65-9
Solubility (25°C)	DMSO 30 mg/mL
Storage	-20°C, protect from light, dry, sealed

References

[1] Lim EJ, et al. Apoptosis. Tunicamycin promotes apoptosis in leukemia cells through ROS generation and downregulation of survivin expression.

[2] Lauer ME, et al. J Biol Chem. Primary murine airway smooth muscle cells exposed to poly(I,C) or tunicamycin synthesize a leukocyte-adhesive hyaluronan matrix.

[3] Duriez M, et al. J Biol Chem. The hepatitis B virus precore protein is retrotransported from endoplasmic reticulum (ER) to cytosol through the ER-associated degradation pathway.