Trichostatin A

Biological Activity

Trichostatin A (TSA) is a potent and specific inhibitor of mammalian histone deacetylase (HDAC) class I/II, with an IC50 value of 1.8 nM for HDAC. Trichostatin A (TSA) is an antifungal antibiotic derived from Streptomyces that inhibits mammalian histone deacetylase (HDAC). TSA inhibits the eukaryotic cell cycle during the beginning of the growth stage. Trichostatin A (TSA) can be used to alter gene expression by interfering with the removal of acetyl groups from histones (histone deacetylases, HDAC) and therefore altering the ability of DNA transcription factors to access the DNA molecules inside chromatin. It is a member of a larger class of histone deacetylase inhibitors (HDIs or HDACIs) that have a broad spectrum of epigenetic activities. Trichostatin A has also been shown to inhibit both G1- and G2-phases of the mammalian cell cycle and has been tested for use as a potential anticancer agent.

Product Citations

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  Cell Death Differ. 2026 Jan 31; .

  Targeting the USP7-PRMT6 epigenetic axis overcomes chemoresistance in breast cancer by coordinating H3R2me2a deposition and RNF168 methylation for DNA
  Trichostatin A purchased from AbMole

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  Sci Immunol. 2025 Oct 03;10(112):eadu6730.

  Vaccination-induced T cell responses maintain polyclonality with high antigen receptor avidity
  Trichostatin A purchased from AbMole

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  iScience. 2025 Feb 05.

  Coronavirus replicase epitopes induce cross-reactive CD8 T cell responses in SARS-CoV-2-naive people with HIV-1
  Trichostatin A purchased from AbMole

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  BioRxiv. 2025 Oct 07;680634.

  TCR activation impairs CAR-T cytotoxicity against separate target cells
  Trichostatin A purchased from AbMole

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  BioRxiv. 2025 Nov 19;688845.

  Antigen-reactive CD4+ T cells after SARS-CoV-2 vaccination show divergent phenotypic states with or without restimulation bias
  Trichostatin A purchased from AbMole

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  BioRxiv. 2025 Feb 06;636644.

  Glycolytic flux sustains human Th1 identity and effector function via STAT1 glycosylation
  Trichostatin A purchased from AbMole

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  Advanced Science. 2024 Aug 13.

  Dissecting the Distinct Tumor Microenvironments of HRD and HRP Ovarian Cancer: Implications for Targeted Therapies to Overcome PARPi Resistance in HRD Tumors and Refractoriness in HRP Tumors
  Trichostatin A purchased from AbMole

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  BioRxiv. 2024 Oct 30;620795.

  Quality of vaccination-induced T cell responses is conveyed by polyclonality and high, but not maximum, antigen receptor avidity
  Trichostatin A purchased from AbMole

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  BioRxiv. 2024 Nov 01.

  Quality of vaccination-induced T cell responses is conveyed by polyclonality and high, but not maximum, antigen receptor avidity
  Trichostatin A purchased from AbMole

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  BioRxiv. 2023 May 28;542585.

  Imaging the microscopic viscoelastic anisotropy in living cells
  Trichostatin A purchased from AbMole

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  bioRxiv. 2023 May 30.

  Imaging the microscopic viscoelastic anisotropy in living cells
  Trichostatin A purchased from AbMole

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  University of Otago. 2019.

  Transcriptional effects of mood stabiliser drugs in a serotonergic cell line
  Trichostatin A purchased from AbMole

Customer Product Validations & Biological Datas

	Source	Oncotarget (2017). Figure 2. Trichostatin A
	Method	Western blotting
	Cell Lines	RLE-6TN cells
	Concentrations	100 nM
	Incubation Time	72 h
	Results	Snail was significantly increased in irradiated group which was effectively modulated by the treatment of TSA, as we observed from the data of western blot

	Source	Oncotarget (2017). Figure 1. Trichostatin A
	Method	Western blotting
	Cell Lines	RLE-6TN cells
	Concentrations	100 nM
	Incubation Time	72 h
	Results	TSA led to a significant modification in the protein and gene expressions of both E-cadherin and α-SMA in cells collected at 72 h after irradation.

Protocol (for reference only)

Cell Experiment
Cell lines	MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3 cell lines
Preparation method	Cell Proliferation Assay. Stock cultures of breast cancer cell lines MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3 were grown in DMEM containing 10% (v/v) FCS, 2 mM L-glutamine, 100 units/ml penicillin, and 100 mg/ml streptomycin at 37°C in 5% CO2 humidified atmosphere. Cells were counted in a hemocytometer after detachment using 0.25% (w/v) trypsin in Dulbecco’s PBS without Ca21 or Mg21 containing 0.02% (w/v) EDTA. Viability was determined by trypan blue exclusion. For each cell line, cells were seeded in 96-well microtiter plates at optimal densities determined in prior experiments to ensure exponential growth for the duration of the assay. After a 24-h preincubation, growth medium was replaced with experimental medium containing TSA at final concentrations ranging from 10-12 M to 10-5 M in log dilutions and 0.1% (v/v) ethanol, or growth medium containing 0.1% (v/v) ethanol as a vehicle control. After 96 h incubation, cell proliferation was estimated using the sulforhodamine B colorimetric assay (11), and the results are expressed as the mean 6 SD for six replicates as a percentage of vehicle control (taken as 100%).
Concentrations	10-12 M to 10-5 M
Incubation time	96 h

Animal Experiment
Animal models	Inbred virgin female (Ludwig/Wistar/ Olac) rats bearing tumors induced with NMU model
Formulation	DMSO
Dosages	500 µg/kg TSA in50 µl DMSO twice weekly for 4 weeks
Administration	s.c. injection

Chemical Information

Molecular Weight	302.37
Formula	C17H22N2O3
CAS Number	58880-19-6
Solubility (25°C)	DMSO 23 mg/mL
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Hu et al. PLoS One. Trichostatin A selectively suppresses the cold-induced transcription of the ZmDREB1 gene in maize.

[2] Choi et al. Clin Exp Allergy. Trichostatin A attenuates airway inflammation in mouse asthma model.

[3] Papeleu et al. J Hepatol. Trichostatin A induces differential cell cycle arrests but does not induce apoptosis in primary cultures of mitogen-stimulated rat hepatocytes.

[4] Vigushin et al. Clin Cancer Res. Trichostatin A is a histone deacetylase inhibitor with potent antitumor activity against breast cancer in vivo.