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Rhodamine B hydrazide is a good sulfite probe with colorless, non-fluorescent properties, which produces fluorescence intensity dependent on sulfite concentration (5-800 ng/mL; detection limit = 1.4 ng/mL (3σ)). Sulfite reduction solubilizes oxygen and generates superoxide radicals, which bind to Rhodamine B hydrazide to form the fluorescent product Rhodamine B. This fluorescence can be enhanced by Tween 80 surfactant micelles.Rhodamine B hydrazide exhibits maximum absorption at 554 nm and maximum fluorescence emission at 574 nm. Rhodamine B hydrazide has maximum absorption at 554 nm and maximum fluorescence emission at 574 nm.
| Molecular Weight | 456.58 |
| Formula | C28H32N4O2 |
| CAS Number | 74317-53-6 |
| Solubility (25°C) | DMSO 90 mg/mL |
| Storage | 4°C, protect from light |
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