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The recombinant s. pyogenes serotype M1 cas9 consists of 1407 amino acids and predicts a molecular mass of 163 kDa.
A DNA sequence encoding the s. pyogenes serotype M1 cas9 (WP_010922251.1) (Met1-Asp1368) was expressed with a nuclear localization sequence (NLS) at the N-terminus, two NLSs and a polyhistidine tag at the C-terminus.
Activity: 1 ug = 5 U; 1 U is defined as cutting 1 ug 1 KB dsDNA in 30 min at 37°C
Endotoxin: < 1.0 EU per μg protein
Formulation: Liquid. Supplied from sterile 20 mM Tris, 300 mM NaCl, 50 % glycerol, pH 8.0, 0.5 mM PMSF, 0.1 mM EDTA, 0.5 mM TCEP.
| Storage | Stable for up to twelve months from date of receipt at -20℃ to -80℃ |
[2] A V Bannikov, et al. Mol Biol (Mosk). [CRISPR/CAS9, the King of Genome Editing Tools]
[5] Le Cong, et al. Methods Mol Biol. Genome engineering using CRISPR-Cas9 system
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