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Recombinant Rat CNTF (E. coli)

Cat. No. M15020

All AbMole products are for research use only, cannot be used for human consumption.

Recombinant Rat CNTF (E. coli) Structure
Synonym:

Ciliary Neurotrophic Factor

Size Price Availability Quantity
10ug USD 155 In stock
50ug USD 385 In stock
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Quality Control & Documentation
  • Purity: >95% , Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
Biological Activity

Species: Rat
Purity: SDS-PAGE, > 95%
Endotoxin: protein < 0.2 EU/μg was determined by gel coagulation
Expression system: E. coli
Apparent molecular weight: ~22.9 kDa, sdS-PAGE under reduction conditions
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles


Biological activity: ED50 < 30.0 ng/ mL, by inducing TF-1 cells to produce alkaline phosphatase. CNTF is an effective survival factor for neurons and oligodendrocytes and may play a role in reducing tissue damage when inflammation increases. Mutations in this gene lead to abnormal splicing, resulting in ciliary neurotrophic factor deficiency, but this phenotype is not causally associated with neurological disease.

Chemical Information
Solubility (25°C) Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
References

[1] Longfu Xu, et al. Protein Expr Purif. Purification and characterization of a long-acting ciliary neurotrophic factor via genetically fused with an albumin-binding domain

[2] Isabelle Cognet, et al. Eur Cytokine Netw. Expression of biologically active mouse ciliary neutrophic factor (CNTF) and soluble CNTFRalpha in Escherichia coli and characterization of their functional specificities

[3] I Saggio, et al. Anal Biochem. Nonradioactive receptor binding assay for ciliary neurotrophic factor

[4] K Ohta, et al. Biochem Mol Biol Int. Tissue expression of rat ciliary neurotrophic factor (CNTF) mRNA and production of the recombinant CNTF

[5] M Thier, et al. J Neurosci Res. Site-directed mutagenesis of human CNTF: functional analysis of recombinant variants

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Keywords: Recombinant Rat CNTF (E. coli), Ciliary Neurotrophic Factor supplier, Cytokines and Growth Factors, inhibitors, activators

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