All AbMole products are for research use only, cannot be used for human consumption.
Species: Rat
Expression system: HEK 293
Purity: 98% by SDS-PAGE analysis
Endotoxin: Protein < 0.2 EU/μg measured by gel coagulation
Apparent molecular weight: ~8.2 kDa, reduction conditions on SDS-PAGE
Storage and Stability: After receipt, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stabilized at 4°C for 1 week or at -20°C for 3 months. For long-term preservation, carrier protein (e.g., 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.
Bioactivity: The EC50 value of rat MIP-3α /CCL20 for Ca2+ mobilization in CHO-K1/ G15/rCCR6 cells (human G15 and rat CCR6 are stably expressed in CHO-K1 cells) was less than 0.6 μg/ml. Mip-3α is expressed in liver, lymph nodes, appendix, PBL and lung and can signal through the CCR6 receptor. It has a strong chemotactic effect on lymphocytes and a weak attractive effect on neutrophils. Mip-3α is involved in the formation and function of mucosal lymphoid tissue through the attraction of lymphocytes and dendritic cells to the epithelial cells surrounding mucosal lymphoid tissue. Promote memory CD4+ T cell adhesion and inhibit bone marrow immature progenitor cell colony formation.
| Solubility (25°C) | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
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All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
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