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IL-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R). IL34 (Interleukin 34) is a Protein Coding gene. IL-34, also known as uncharacterized protein C16 or f77 homolog, belongs to the IL-34 family. IL-34 increases the growth or survival of immune cells known as monocytes. Besides, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures. IL-34 protein is expressed in various tissues, including heart, brain, lung, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and most abundant in the spleen.
The recombinant human IL34 consists of 233 amino acids and predicts a molecular mass of 26.7 kDa. A DNA sequence encoding the human IL34 (AAH29804.1) (Met1-Pro242) was expressed with a polyhistidine tag at the C-terminus.
Lyophilized from sterile PBS, pH 7.4.
Measured in a cell proliferation assay using human peripheral blood mononuclear cell, the ED50 is 2-8 ng/mL.
| Form | Lyophilized powder |
| Solubility (25°C) | Reconstitute the lyophilized powder in distilled water up to 100 μg/ml. |
| Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
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