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Recombinant Human Fibronectin
Concentration 1mg/ml
| Cell Experiment | |
|---|---|
| Cell lines | C57BL/6 mice dermal FCs |
| Preparation method | Different concentrations of RHC and rhFN (concentration to 0.01 μM, 0.1 μM, 1 μM, 10 μM, respectively) as well as equivalent phosphate buffer saline (PBS) were completely immersed in DMEM media supplemented and 1% (v/v) penicillin/streptomycin. The dermal FCs were inoculated in 96-well plates (3×10^3 cells/well), and then added 100 µl of the above four media with 10% FBS. Afterward, some cells in 96-well plates were culture at a specific point in time (24 h) at 37°C in a 5% CO2 humidified incubator. |
| Concentrations | 0.01, 0.1, 1, 10 μM |
| Incubation time | 24 h |
| Animal Experiment | |
|---|---|
| Animal models | C57BL/6 mice |
| Formulation | Saline |
| Dosages | 1 μM |
| Administration | apply |
| Solubility (25°C) | Water Saline PBS |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
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