Puromycin dihydrochloride

Biological Activity

Puromycin dihydrochloride is a protein synthesis inhibitor. Puromycin 2HCl leads to the premature release of polypeptide chains as polypeptidyl purine derivatives. Analog of the 3' end of aminoacyl-tRNA. Puromycin dihydrochloride Inhibits translation in both in vitro and in vivo systems. Puromycin dihydrochloride inhibits the transport of proteins into the mitochondria in vitro. Puromycin inhibits protein import into mitochondria by interfering with an ATP-dependent step in the import process and that the ATP-dependent component in the reaction is located inside the inner mitochondrial membrane.

Product Citations

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  Acta Pharmacol Sin. 2026 Jan 16; .

  A sinomenine derivative protects life-threatening inflammatory injuries via covalently binding to a novel allosteric inhibition site of IRF3
  Puromycin dihydrochloride purchased from AbMole

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  Int Immunopharmacol. 2026 Feb 05;173:116302.

  Polysialylated CD56 drives immune evasion in clear cell renal cell carcinoma via engagement of the Siglec-7 checkpoint on CD8+ T cells
  Puromycin dihydrochloride purchased from AbMole

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  Oncol Res. 2026;1900-01-01 10:22:00.000.

  Esketamine Enhances the Chemosensitivity of Colorectal Adenocarcinoma Cells to 5-Fluorouracil via AMPK/mTOR/HMMR Signaling Pathway
  Puromycin dihydrochloride purchased from AbMole

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  Adv Sci (Weinh). 2025 Jun 20; .

  NPR1 Promotes Lipid Droplet Lipolysis to Enhance Mitochondrial Oxidative Phosphorylation and Fuel Gastric Cancer Metastasis
  Puromycin dihydrochloride purchased from AbMole

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  J Biochem Mol Toxicol. 2025 Jul 16;39(8):e70398.

  D‐Mannose Upregulates Testin via the NF‐κB Pathway to Inhibit Breast Cancer Proliferation
  Puromycin dihydrochloride purchased from AbMole

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  BioRxiv. 2025 May 27;656475.

  An Adipose mTORC1 Effector Links Nutrient-induced Ribosomal Protein Translation to Lifespan in Drosophila
  Puromycin dihydrochloride purchased from AbMole

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  Biochim Biophys Acta Mol Basis Dis. 2024 Jul 15;1870(7):167355..

  A novel HOIP frameshift variant alleviates NF-kappaB signalling and sensitizes cells to TNF-induced death
  Puromycin dihydrochloride purchased from AbMole

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  Biochim Biophys Acta Mol Basis Dis. 2024 Oct 25.

  TMEM106B knockdown exhibits a neuroprotective effect in Parkinson's disease models via regulating autophagy-lysosome pathway
  Puromycin dihydrochloride purchased from AbMole

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  J Ethnopharmacol. 2023 Feb 10;302(Pt A):115897.

  Total glucosides of paeony alleviates scleroderma by inhibiting type I interferon responses
  Puromycin dihydrochloride purchased from AbMole

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  Transl Oncol. 2023 Feb;28;101617.

  KIAA1199 promotes oxaliplatin resistance and epithelial mesenchymal transition of colorectal cancer via protein O-GlcNAcylation
  Puromycin dihydrochloride purchased from AbMole

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  Nucleic Acids Res. 2021 Mar 18;49(5):2848-2858.

  HuB and HuD repress telomerase activity by dissociating HuR from TERC
  Puromycin dihydrochloride purchased from AbMole

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  Int J Chron Obstruct Pulmon Dis. 2021 Jun 4;16:1661-1675.

  Histone Deacetylase 2 Suppresses Skeletal Muscle Atrophy and Senescence via NF-κB Signaling Pathway in Cigarette Smoke-Induced Mice with Emphysema
  Puromycin dihydrochloride purchased from AbMole

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  DNA Cell Biol. 2021 Jul;40(7):895-905.

  AEG-1 Knockdown Sensitizes Glioma Cells to Radiation Through Impairing Homologous Recombination Via Targeting RFC5
  Puromycin dihydrochloride purchased from AbMole

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  Nature. 2020 Dec;588(7839):664-669.

  Creation of bladder assembloids mimicking tissue regeneration and cancer
  Puromycin dihydrochloride purchased from AbMole

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  Cell Death Dis. 2020 Oct 23;11(10):902.

  The miR-30a-5p/CLCF1 axis regulates sorafenib resistance and aerobic glycolysis in hepatocellular carcinoma
  Puromycin dihydrochloride purchased from AbMole

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  Environ Health Perspect. 2020 Jun;128(6):67008.

  Effects of Inorganic Arsenic on Human Prostate Stem-Progenitor Cell Transformation, Autophagic Flux Blockade, and NRF2 Pathway Activation
  Puromycin dihydrochloride purchased from AbMole

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  Exp Biol Med (Maywood). 2020 Jun;245(11):925-932.

  Circular RNA circ-CDYL sponges miR-1180 to elevate yes-associated protein in multiple myeloma
  Puromycin dihydrochloride purchased from AbMole

Protocol (for reference only)

Cell Experiment
Cell lines	SH-SY5Y cells
Preparation method	To stably silence HuR, HuB, or HuD, SH-SY5Y cells were infected with lentiviruses bearing vector expressing shHuR, shHuD or shHuB. Forty-eight hours later, cells were cultured in medium containing puromycin (1.5 μg/ml) and cultured for an additional 7 days. Cells then were cultured in medium containing puromycin (1.0 μg/ml) until cells were collected for experiments.
Concentrations	1.5 μg/mL, 1.0 μg/mL
Incubation time	7 days

Animal Experiment
Animal models	Vitamin D receptor null mutant mice
Formulation	PBS
Dosages	0.040 μmol/g
Administration	Intraperitoneal injection

Chemical Information

Molecular Weight	544.43
Formula	C22H29N7O5.2HCl
CAS Number	58-58-2
Solubility (25°C)	Water ≥ 30 mg/mL
Storage	-20°C, dry, sealed

References

[1] Price J, et al. Biochim Biophys Acta. Puromycin inhibits protein import into mitochondria by interfering with an intramitochondrial ATP-dependent reaction.