All AbMole products are for research use only, cannot be used for human consumption.
PKH-26 is a red fluorescent dye, PKH-26 can stably bind to the lipid region of cell membrane and emit red fluorescence (Ex/Em=551/567 nm), which is mainly used for in vitro cell labeling, in vitro cell proliferation studies and in vivo and in vitro cell tracing studies.
PKH-26 Exosome Staining Protocol (for reference only):
A PKH-26 kit was employed to label Exo according to the manufacturer's protocol. Typically, the Exo was incubated with 100 μM PKH-26 dye solution (50 μL of PKH-26 dye solution per 100 μg of Exo) at 37 °C for 10 min in a dark environment. Subsequently, 20 mL of PBS was added to stop the staining. The PKH-26-labeled Exo was obtained through differential centrifugation and resuspended with 500 μL of PBS.
https://pubmed.ncbi.nlm.nih.gov/34823355/
Small. 2025 Sep 29; .
Spheroid‐Derived BMSC Exosomes Enhance Proliferation and Matrix Remodeling of Nucleus Pulposus Cell Clusters in Degenerative Disc Repair
PKH-26 (red) purchased from AbMole
Research (Wash D C). 2025 Nov 16;8:1019.
Cabbage Exosome-Like Nanoparticles Encapsulating Small Noncoding tsRNA Prevent Postinjury Arterial Restenosis.
PKH-26 (red) purchased from AbMole
Exp Cell Res. 2024 Feb;113960.
Exosomes derived from human umbilical cord mesenchymal stem cells pretreated by monosialoteterahexosyl ganglioside alleviate intracerebral hemorrhage by down-regulating autophagy
PKH-26 (red) purchased from AbMole
| Cell Experiment | |
|---|---|
| Cell lines | MeR190 and MeI493 cells |
| Preparation method | The cells are then resuspended in a loading buffer and incubated for 40 min with freshly prepared PKH-26 (stock 1 mM in ethanol) at room temperature. For the loading of tumor cells, it is recommended to use 0.8 μl, for small cells 0.5 μl PKH-26 in 200 μl loading buffer. |
| Concentrations | Not mentioned |
| Incubation time | 40 min |
| Animal Experiment | |
|---|---|
| Animal models | |
| Formulation | |
| Dosages | |
| Administration | |
| Molecular Weight | 961.32 |
| Formula | C59H97IN2 |
| CAS Number | 154214-55-8 |
| Solubility (25°C) | Ethanol |
| Storage | 2-8°C, protect from light, sealed, stable for 6-12 months |
| Related Fluorescent Dye Products |
|---|
| Carminic Acid (Natural dye)
Carminic acid is an anthraquinone red dye. Carminic acid is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. |
| Guanidine hydrochloride
Guanidine hydrochloride, the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant. |
| Indocyanine green
Indocyanine green(Cardiogreen) is a cyanine dye used in medical diagnostics. It is used for determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography. |
| CY7
Cy7 (Sulfo-Cyanine7) is a fluorescence labeling agent (Ex=750 nm, Em=773 nm), used to label proteins, antibodies, peptides, and oligonucleotides. |
| Fluo-4 AM
Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm). |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
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