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Phorbol 12-myristate 13-acetate

Cat. No. M4647

All AbMole products are for research use only, cannot be used for human consumption.

Phorbol 12-myristate 13-acetate Structure
Synonym:

PMA, TPA, 12-O-tetradecanoyl-phorbol-13-acetate; 12,13-tetradecanoyl phorbol acetate

Size Price Availability Quantity
1mg USD 44 In stock
2mg USD 80 In stock
5mg USD 110 In stock
10mg USD 160 In stock
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Biological Activity

Phorbol 12-myristate 13-acetate (PMA) is a dual SphK and protein kinase C (PKC) activator, increases the intracellular Ca2+ concentration ([Ca2+]i) in a dose-dependent manner, with an EC50 of 11.7 nM. Phorbol 12-myristate 13-acetate (PMA) induces differentiation in THP-1 cells (80 ng/ml, 24h). Phorbol 12-myristate 13-acetate is also a NF-κB activator.

THP-1 cells were seeded into 6-well cell culture plates at 1×105 cells/ml, 5×105 cells/ml, 1×106 cells/ml and 5×106 cells/ml and induced with PMA of 80 ng/ml. Cells were collected at 12h, 24h, and 36h, respectively, and samples with different cell seeding densities corresponding to different induction times were detected by flow cytometry. Two-way ANOVA analysis showed that there was a significant difference in the positive rate of CD14 molecules between groups with different cell seeding densities, while the CD14 positive rate of 5×105 cells/ml group (41.4%±8.8%) was higher than that of other seeding density groups. Meanwhile, there was significant difference in CD14 positive rate between different induction time groups, and the positive rate of CD14 was higher in group with induction time of 24h (36.7%±11.7%) than other induction time groups.

Product Citations
Protocol (for reference only)
Cell Experiment
Cell lines THP-1 cells
Preparation method The culture conditions for THP-1 cells were the same as for NPC cells, except for the addition of β-mercaptoethanol. THP-1 cells were differentiated into macrophages by incubation with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) (M4647, Abmole, USA) for 48 h.
Concentrations 100 ng/mL
Incubation time 48 h
Animal Experiment
Animal models Male Wistar rat
Formulation Saline
Dosages 100, 200, and 500 μg/kg
Administration Intraperitoneal (i.p.) injection
Chemical Information
Molecular Weight 616.83
Formula C36H56O8
CAS Number 16561-29-8
Solubility (25°C) DMSO 90 mg/mL
Ethanol 90 mg/mL
Storage -20°C, protect from light
References

[1] Tiezhu Liu, et al. PLoS One. Optimization of differentiation and transcriptomic profile of THP-1 cells into macrophage by PMA

[2] Mitsuhiro Kinoshita, et al. Phorbol-12-myristate 13-acetate inhibits Nephronectin gene expression via Protein kinase C alpha and c-Jun/c-Fos transcription factors

[3] Mancy Tong, et al. Methods Mol Biol. Visualization and Quantification of Neutrophil Extracellular Traps

[4] Sukkum Ngullie Chang, et al. Phorbol 12-Myristate 13-Acetate Induced Toxicity Study and the Role of Tangeretin in Abrogating HIF-1α-NF-κB Crosstalk In Vitro and In Vivo

[5] Yoon-Hee Cheon, et al. Eupatilin downregulates phorbol 12-myristate 13-acetate-induced MUC5AC expression via inhibition of p38/ERK/JNK MAPKs signal pathway in human airway epithelial cells

[6] Derya Biriken, et al. Investigation of cytokine and midkine responses of human THP-1 leukemia cells induced by phorbol-12-Myristate-13-Acetate (PMA) at different concentrations and times

[7] Tregei Starr, et al. The phorbol 12-myristate-13-acetate differentiation protocol is critical to the interaction of THP-1 macrophages with Salmonella Typhimurium

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Keywords: Phorbol 12-myristate 13-acetate, PMA, TPA, 12-O-tetradecanoyl-phorbol-13-acetate; 12,13-tetradecanoyl phorbol acetate supplier, PKC, inhibitors, activators

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