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O-Propargyl-Puromycin, an alkyne analog of puromycin, is a potent protein synthesis inhibitor.
| Cell Experiment | |
|---|---|
| Cell lines | HeLa cells |
| Preparation method | To visualize OP-Puro incorporation, we typically exposed cells to culture medium containing 10 μM OP-Puro and 10 μM MG132 for 4 h at 37 °C. |
| Concentrations | 10 μM |
| Incubation time | 4 h |
| Animal Experiment | |
|---|---|
| Animal models | C57BL/6N mice |
| Formulation | Not mentioned |
| Dosages | 50 mg/kg |
| Administration | intraperitoneally injected 30 minutes before the animals were euthanized |
| Molecular Weight | 495.53 |
| Formula | C24H29N7O5 |
| CAS Number | 1416561-90-4 |
| Solubility (25°C) | DMSO ≥ 30 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
[3] Lorena Hidalgo San Jose, et al. Cell-type-specific quantification of protein synthesis in vivo
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