MHY1485

Biological Activity

MHY1485 induced mTOR activation and correspondingly showed a higher docking score than PP242, a well-known ATP-competitive mTOR inhibitor, in docking simulation. In conclusion, MHY1485 has an inhibitory effect on the autophagic process by inhibition of fusion between autophagosomes and lysosomes leading to the accumulation of LC3II protein and enlarged autophagosomes. MHY1485 also induces mTOR activity, providing a possibility for another regulatory mechanism of autophagy by the MHY compound. MHY1485 treatment increases ribosomal protein S6 kinase (S6K) and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) phosphorylation, which are downstream targets of mTOR complex 1 (mTORC1), but decreases phosphorylation of Akt on mTOR complex 2 (mTORC2) target site serine 473.

Product Citations

• 

  Int Immunopharmacol. 2024 Sep 21;142(Pt B):113196.

  Salidroside treatment decreases the susceptibility of atrial fibrillation in diabetic mice by reducing mTOR-STAT3-MCP-1 signaling and atrial inflammation
  MHY1485 purchased from AbMole

• 

  Front Pharmacol. 2024 Jul 26;15:1434568.

  Huanshaodan regulates microglial glucose metabolism reprogramming to alleviate neuroinflammation in AD mice through mTOR/HIF-1a signaling pathway
  MHY1485 purchased from AbMole

• 

  Chemical Engineering Journal. 2024 Apr 09.

  Novel nano-platinum induces autophagy through dual pathways in the treatment of osteosarcoma in cell lines with different P53 expression patterns
  MHY1485 purchased from AbMole

• 

  Heliyon. 2023 Jul;9(7):e17551.

  Itaconate attenuates autoimmune hepatitis via PI3K/AKT/mTOR pathway-mediated inhibition of dendritic cell maturation and autophagy
  MHY1485 purchased from AbMole

• 

  Heliyon. 2023 Jun 22;9(7):e17551.

  Itaconate attenuates autoimmune hepatitis via PI3K/AKT/mTOR pathway-mediated inhibition of dendritic cell maturation and autophagy
  MHY1485 purchased from AbMole

• 

  Int J Neurosci. 2022 Jul 18;1-9.

  Sinomenine exerts a neuroprotective effect on PD mouse model through inhibiting PI3K/AKT/mTOR pathway to enhance autophagy
  MHY1485 purchased from AbMole

• 

  Fish Shellfish Immunol. 2020 Jul;102:480-488.

  Target of rapamycin signaling inhibits autophagy in sea cucumber Apostichopus japonicus
  MHY1485 purchased from AbMole

• 

  Life Sci. 2020 Jun 15;251:117595.

  The in vitro effects of phospholipase D1-mTOR axis in liver fibrogenesis
  MHY1485 purchased from AbMole

Customer Product Validations & Biological Datas

	Source	Life Sciences (2020 Jun). Figure 5. MHY1485 (Abmole Bioscience, US)
	Method	Proliferation analysis by CCK-8 method
	Cell Lines	Rat HSCs cell line
	Concentrations	5 μM
	Incubation Time	48 h
	Results	Moreover, both PLD1-I and PLD1-SiRNA could significantly blunt proliferation of HSC-T6 treated with MHY1485 (mTOR activator)

Protocol (for reference only)

Cell Experiment
Cell lines	CT26 and LLC cells
Preparation method	The number of cells was counted with a Countess II FL. According to a previous report, the cells were plated in culture flasks or dishes at Day 0; 10 μM of MHY1485 or vehicle control (DMSO) were added on Day 1 (24 h after cell plating); 6 Gy X-ray irradiation was delivered on Day 2 (24 h after drug treatment) and the culture medium and cells were collected for experiments on Day 3 (24 h after irradiation).
Concentrations	10 μM
Incubation time	24 h

Animal Experiment
Animal models	C57BL/6J mice
Formulation	DMSO and Saline
Dosages	2 mg/kg
Administration	Intraperitoneally injected

Chemical Information

Molecular Weight	387.39
Formula	C17H21N7O4
CAS Number	326914-06-1
Solubility (25°C)	DMSO 7 mg/mL (Need ultrasonic and warming)
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Jilong Zhou, et al. Cell Death Dis. Administration of follicle-stimulating hormone induces autophagy via upregulation of HIF-1α in mouse granulosa cells

[2] Cheng Y, et al. PLoS One. Promotion of ovarian follicle growth following mTOR activation: synergistic effects of AKT stimulators.

[3] Choi YJ, et al. PLoS One. Inhibitory effect of mTOR activator MHY1485 on autophagy: suppression of lysosomal fusion.