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Macrophage colony stimulating factor (M-CSF), also known as colony stimulating factor-1 (CSF-1), is a hematopoietic growth factor. It can stimulate the survival, proliferation and differentiation of mononuclear phagocytes, and can stimulate the proliferation and movement of macrophages. M-csf is mainly produced by monocytes, macrophages, fibroblasts and endothelial cells. The interaction of M-CSF with its receptor C-FMS is associated with growth, invasion, and metastasis in a variety of diseases, including breast cancer and endometrial cancer.
Measured in a cell proliferation assay using Murine M-NFS-60 cells, the ED50 is < 3.0 ng/ml, corresponding to a specific activity of > 3.3 × 105 units/mg.
Accession # P07141
Protein Construction: M-CSF (Lys33-Pro187)
Endotoxin: < 0.2 EU/μg
Apparent Molecular Weight: ~30 kDa
Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0.
| Form | Lyophilized powder |
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH2O up to 100 μg/ml. |
| Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
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