All AbMole products are for research use only, cannot be used for human consumption.
JNJ-10229570 (UNII-N9IX402L35) is is a novel melanocortin receptor 1 (MC1R) and melanocortin receptor 5 (MC5R) antagonist, which inhibits the binding of 125I-NDP-α-MSH to cells expressing human MC1R and MC5R with IC50 values of 270 nM and 200 nM, respectively. JNJ-10229570 dose dependently inhibits the production of sebaceous lipids in cultured primary human sebocytes.
In vivo, topical treatment with JNJ-10229570 of human skins transplanted onto SCID mice result in a marked decrease in sebum-specific lipid production, sebaceous gland's size and the expression of the sebaceous differentiation marker epithelial-membrane antigen (EMA). Topical treatment with 0.05% JNJ-10229570 leads to a distinct reduction in both the steady-state and the newly-synthesized sebum-specific lipids, with lesser effects on triglycerides and cholesterol.
| Cell Experiment | |
|---|---|
| Cell lines | differentiated sebocytes |
| Preparation method | Nile red assay. Briefly, differentiated sebocytes grown in 96-well plates were treated with or without 1 uM of JNJ-10229570 (8 repeats, 8 days), washed once, incubated in 1 uM Nile red and 0.001% Pluronic F127 (4 h, RT, dark), and washed again. Fluorescence (485 nm excitation, 565 nm emission) was determined in a SPECTRAmax Gemini-EM plate reader (Molecular Devices, Sunnyvale, CA) by SoftMax Pro program. |
| Concentrations | 1 uM |
| Incubation time | - |
| Animal Experiment | |
|---|---|
| Animal models | Human skins transplanted onto SCID mice |
| Formulation | dissolved in a vehicle of ethanol: propylene glycol (7:3) |
| Dosages | JNJ-10229570 (0.05%) 20 ml/1.5 cm2 |
| Administration | applied onto each skin xenograft once daily, 5 days/week, for 30-34 treatments |
| Molecular Weight | 389.47 |
| Formula | C22H19N3O2S |
| CAS Number | 524923-88-4 |
| Solubility (25°C) | DMSO 60 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Related Melanocortin Receptor Products |
|---|
| α-MSH
α-MSH (α-Melanocyte-stimulating hormone) is a tridecapeptide, mostly produced by the cells in the brain, pituitary and circulation. |
| Melanotan I acetate
Melanotan I acetate is a synthetic analogue of α-melanocyte stimulating hormone (α-MSH). *This compound is unstable in solutions, freshly prepared is recommended. |
| Setmelanotide
Setmelanotide is a selective agonist of the MC4 receptor, with EC50 values of 0.27 nM and 0.28 nM for human and rat MC4R, respectively. |
| Bremelanotide Acetate
Bremelanotide Acetate (PT-141 Acetate) is a peptide containing seven amino acids and is also a macrocyclic compound. In addition, Bremelanotide Acetate is an agonist of the melanocortin 4 receptor (MC4R). |
| β-Melanocyte Stimulating Hormone TFA, human
β-Melanocyte Stimulating Hormone TFA, human is a 22-residue peptide, which acts as an endogenous melanocortin-4 receptor (MC4-R) agonist. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2026 AbMole BioScience. All Rights Reserved.
