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Hygromycin B is a selective antibiotic that is effective on most bacteria, fungi and higher eukaryotes, inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S ribosome.
J Med Chem. 2023 Dec;17138–17154.
All-Hydrocarbon Stapled Peptide Multifunctional Agonists at Opioid and Neuropeptide FF Receptors: Highly Potent, Long-Lasting Brain Permeant Analgesics with Diminished Side Effects
Hygromycin B purchased from AbMole
| Cell Experiment | |
|---|---|
| Cell lines | Chinese hamster ovary (CHO) cells |
| Preparation method | Chinese hamster ovary (CHO) cells (CHOMOPr, CHODOPr, CHOKOPr, CHONPFF1R and HEK293NPFF2R) stably expressing the C-terminally modified Gαq15 were cultured in Ham’s F12 media containing 10% FBS, 100 μg/mL streptomycin, 100 IU/mL penicillin, 100 μg/mL hygromycin B and 200 μg/mL zeocin. |
| Concentrations | 100 μg/mL |
| Incubation time | Not mentioned |
| Animal Experiment | |
|---|---|
| Animal models | Experimental meningoencephalitis mice model |
| Formulation | Saline |
| Dosages | 20 mg/kg |
| Administration | Intraperitoneally injected |
| Molecular Weight | 527.52 |
| Formula | C20H37N3O13 |
| CAS Number | 31282-04-9 |
| Solubility (25°C) | Water 50 mg/mL DMSO 50 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
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|---|
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