Hexadimethrine bromide

Biological Activity

Hexadimethrine bromide can be used to transfect mammalian cells with DNA. It can be used to increase the efficiency of lipofection transfections. Hexadimethrine bromide has been used for lentivirus infection in cells. It has been used for infection of cells with retroviral supernatant.

Enhanced Transfection of Mammalian Cells with Polybrene (for reference only)
Polybrene (Cat. No. M9703) is supplied as a powder/solid and is soluble in water (up to 100 mg/mL). To generate a working solution, Polybrene should first be dissolved in 1 mL sterile water to generate a 10 mg/mL stock solution.
Polybrene working solution is generated from the polybrene stock solution (10 mg/mL in sterile water). This stock solution can be further diluted in the culture media to generate the desired working concentration for the transfection or transduction media.

Step 1: Prepare DNA Plasmid
Sufficient plasmid DNA must first be generated. Begin by amplifying the quantity of the plasmid by performing bacterial transformation. Grow the transformed bacterial culture overnight (in 37°C shaker) and extract the plasmid DNA using DNA MAXIprep kit.
Step 2: Prepare HEK Cells
Culture HEK293T cells in HEK media (DMEM/F-12 media, FBS, non-essential amino acids, L-glutamine) within humidified incubators (standard conditions of 37˚C, 5% CO2 and 21% O2). Once the culture has reached high confluency (>90%), the cells are ready for transfection.
Step 3: Prepare Transfection Media
Immediately before transfection, replace the HEK culture media with DMEM.
Prepare the transfection media, which consists of an optimized ratio of DMEM, DNA plasmid, lipofection reagent and polybrene stock solution. Typically, polybrene is used at a concentration of 4 µg/mL - 10 µg/mL.
Step 4: Transfection
Gently mix the transfection media before adding dropwise to the culture plate, to prevent damaging the cells.
Place the cells immediately in the incubator with the following settings overnight: 35˚C, 3% CO2 and 21% O2.
Step 5: Harvesting
Return culture to standard maintenance incubator. Leave the cells for 48-72 h post-transfection before proceeding to downstream application (i.e., harvesting of virus from media).

Product Citations

• 

  Biochem Pharmacol. 2025 Jan 05.

  Peptide-based CAR-NK cells: A novel strategy for the treatment of solid tumors
  Hexadimethrine bromide purchased from AbMole

• 

  Cancer Lett. 2024 Apr 6;590:216861.

  TRAF6 enhances PD-L1 expression through YAP1-TFCP2 signaling in melanoma
  Hexadimethrine bromide purchased from AbMole

• 

  ACS Biomater Sci Eng. 2023 Apr 12.

  TNF-R1 Cellular Nanovesicles Loaded on the Thermosensitive F-127 Hydrogel Enhance the Repair of Scalded Skin
  Hexadimethrine bromide purchased from AbMole

• 

  BioRxiv. 2022 Sep 28;509909.

  TRAF6 modulates PD-L1 expression through YAP1-TFCP2 signaling in melanoma
  Hexadimethrine bromide purchased from AbMole

• 

  bioRxiv. 2022 Sep 28.

  TRAF6 modulates PD-L1 expression through YAP1-TFCP2 signaling in melanoma
  Hexadimethrine bromide purchased from AbMole

Chemical Information

Molecular Weight	1122.6
Formula	C39H90Br6N6X2
CAS Number	28728-55-4
Solubility (25°C)	Water 100 mg/mL
Storage	2-8°C, protect from light, dry, sealed

References

[1] Paul Lin, et al. PLoS One. Polybrene inhibits human mesenchymal stem cell proliferation during lentiviral transduction

[2] F Jacobsen, et al. J Gene Med . Polybrene improves transfection efficacy of recombinant replication-deficient adenovirus in cutaneous cells and burned skin

[3] Howard E Davis, et al. Biophys Chem . Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on target cell membranes