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HBC530 is a GFP fluorophore-like synthetic dye, with a structurally rigid electron acceptor and a strong electron donor. HBC is nonfluorescent in solution, and when combined with Pepper (RNA aptamer), HBC forms a tight complex and activates and emits bright fluorescence (Kd of ~3.5 nM). HBC emission peaks vary in different complexes and covers the spectrum from cyan to red. HBC can be used in the live cell imaging of RNA (Em/Ex = 530/485 nm).
| Molecular Weight | 303.36 |
| Formula | C19H17N3O |
| CAS Number | 156840-13-0 |
| Form | Solid |
| Solubility (25°C) | DMSO 65 mg/mL |
| Storage | 4°C, protect from light |
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