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H2DCFDA

Cat. No. M9096

All AbMole products are for research use only, cannot be used for human consumption.

H2DCFDA Structure
Synonym:

DCFH-DA; 2',7'-Dichlorodihydrofluorescein diacetate

Size Price Availability Quantity
5mg USD 20 In stock
10mg USD 25 In stock
25mg USD 40 In stock
50mg USD 50 In stock
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Biological Activity

H2DCFDA is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm). H2DCFDA can be used for the Bacterial reactive oxygen species (ROS) production assay.

ROS Measurements

For the detection of intracellular ROS level ,ROS-sensitive probe H2DCFDA is used. Adherent cells (ESCs, difESCs, eMSCs, HeLa, U118) are incubated with 5 µM staining solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer. Lymphocytes, both control and PHA-activated, are resuspended in PBS, incubated with 5 µM of H2DCFDA in the dark for 30 min at 37°C, and immediately analyzed. Along with the H2DCFDA probe, if indicated, ROS-insensitive modification of the fluorescent dye DCFDA is used. The staining procedure is the same as for the H2DCFDA.

Product Citations
Customer Product Validations & Biological Datas
Source Comp Biochem Physiol C Toxicol Pharmacol (2021 Apr), Figure 3, H2DCFDA (Abmole Bioscience)
Method measure the ROS levels
Cell Lines Each C. elegans strain
Concentrations 50 μM
Incubation Time 1 h
Results The results showed that CEO exposure made no significant difference to the GSH amount in each worm, however, the lipid peroxidation degree decreased due to the reduced ROS levels.
Protocol (for reference only)
Cell Experiment
Cell lines Nucleus pulposus cells (NPCs)
Preparation method H2DCFDA （Abmole, M9096）was used to detect ROS. After the cell intervention, the suspension cells were digested with pancreatic enzymes. The cells were thoroughly washed with PBS, and a 5 μM H2DCFDA working solution was added and incubated at 37°C for 30 min away from light. The supernatant was centrifuged and washed with PBS for flow cytometry analysis.
Concentrations 5 μM
Incubation time 30 min
Animal Experiment
Animal models
Formulation
Dosages
Administration
Chemical Information
Molecular Weight 487.29
Formula C24H16Cl2O7
CAS Number 4091-99-0
Solubility (25°C) DMSO: ≥ 90 mg/mL
Storage -20°C, protect from light
References

[1] Karyne Rangel, et al. Microorganisms. Detrimental Effect of Ozone on Pathogenic Bacteria

[2] Shijing Liao, et al. Int J Nanomedicine. Antibacterial activity and mechanism of silver nanoparticles against multidrug-resistant Pseudomonas aeruginosa

[3] Lyublinskaya OG, et al. Redox Biol. Redox environment in stem and differentiated cells: A quantitative approach.

[4] Nicky Pirotte, et al. Oxid Med Cell Longev. Reactive Oxygen Species in Planarian Regeneration: An Upstream Necessity for Correct Patterning and Brain Formation

[5] Carole Gauron, et al. Sci Rep. Sustained production of ROS triggers compensatory proliferation and is required for regeneration to proceed

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Keywords: H2DCFDA, DCFH-DA; 2',7'-Dichlorodihydrofluorescein diacetate supplier, Fluorescent Dye, inhibitors, activators

All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.


 
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