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Freund′s Adjuvants may be used to produce water-in-oil emulsions of immunogens. Antigens in water-in-oil emulsions stimulate high and long-lasting antibody responses which can be attributed to the slow release of antigen.
Freund′s adjuvant (incomplete) lacks the Mycobacteria found in Complete Freund′s Adjuvant so it minimizes the side-effects. It is used for boost injections. It was used to emulsify antigens that are injected into animals to produce monoclonal antibodies from the antiserum.
Each mL contains 0.85 mL paraffin oil and 0.15 mL mannide monooleate.
| Solubility (25°C) | Antigens (preferably in saline) are typically mixed with an equal volume of the adjuvant to form an emulsion. |
| Storage | 2-8°C, protect from light, sealed. Do not freeze! |
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