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FITC-NHS emits green fluorescence with an excitation wavelength around 488 nm and an emission wavelength around 520 nm. This allows differentiation from long-wavelength dyes (such as Cy5) in multicolour fluorescence systems, facilitating co-localisation and multi-channel analysis.
Applications
1. Labelling of biomolecules and lipid systems
FITC-NHS can be employed for surface labelling of proteins, peptides, liposomes, and nanoparticles, enabling visual tracking.
2. Multicolour fluorescence experiments
Its green fluorescence can be combined with long-wavelength dyes such as Cy3 and Cy5 to achieve multichannel colocalisation analysis.
3. Interface functionalisation
Through terminal chemical reactions, stable fluorescent interfaces can be constructed on liposome or nanoparticle surfaces, providing a foundation for subsequent surface modification or functionalisation experiments.
4. Quantitative analysis and tracking
Fluorescence signal intensity correlates with labelled molecule quantity, enabling assessment of molecular distribution, particle coverage, and dynamic behaviour analysis.
| Solubility (25°C) | DMSO |
| Storage | -20°C, protect from light, dry, sealed |
| Related Fluorescent Dye Products |
|---|
| Carminic Acid (Natural dye)
Carminic acid is an anthraquinone red dye. Carminic acid is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. |
| Guanidine hydrochloride
Guanidine hydrochloride, the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant. |
| Indocyanine green
Indocyanine green(Cardiogreen) is a cyanine dye used in medical diagnostics. It is used for determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography. |
| CY7
Cy7 (Sulfo-Cyanine7) is a fluorescence labeling agent (Ex=750 nm, Em=773 nm), used to label proteins, antibodies, peptides, and oligonucleotides. |
| Fluo-4 AM
Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm). |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
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