Dihydrotestosterone

Biological Activity

Dihydrotestosterone is a hormone with powerful androgenic actions, causes the body to mature during puberty and is responsible for many of the physical characteristics associated with adult males. It can be used to construct animal models of polycystic ovary syndrome (PCOS).

Product Citations

• 

  Adv Healthc Mater. 2026 Feb 21;e05171.

  Macrophage Membrane‐Cloaked, ROS‐Triggered Quercetin Nanocarriers Target Ovarian Lesions to Treat Polycystic Ovary Syndrome
  Dihydrotestosterone purchased from AbMole

• 

  Cell Biosci. 2025 Oct 29;15(1):148.

  Sexual dimorphism in colon is mediated by an androgen-IL33+ stromal cell axis
  Dihydrotestosterone purchased from AbMole

• 

  FASEB J. 2025 Jul 15;39(13):e70820.

  Single‐Cell RNA Sequencing Highlights a Contribution of Human Amniotic Mesenchymal Stem Cells‐Derived Exosomes to Androgenetic Alopecia
  Dihydrotestosterone purchased from AbMole

• 

  J Inflamm Res. 2025 Nov 13;18:15911-15924.

  Sex Differences and Androgen Regulation Shape Mucosal Immune Phenotype, Gut Microbiota, and Microbiota-Derived Lactate in SKG Arthritis Model.
  Dihydrotestosterone purchased from AbMole

• 

  Low Urin Tract Symptoms. 2025 May 13;17(3):e70015.

  Saw Palmetto Extract Ameliorates Benign Prostatic Hyperplasia by Regulating 5α‐Reductase and Apoptosis In Vitro and In Vivo
  Dihydrotestosterone purchased from AbMole

• 

  Am J Pathol. 2024 Sep 05;S0002-9440(24)00331-6.

  Testosterone-induced H3K27 deacetylation participates in granulosa cell proliferation suppression and pathogenesis of Polycystic Ovary Syndrome
  Dihydrotestosterone purchased from AbMole

• 

  Mol Cell Endocrinol. 2024 Sep 26.

  TIGAR relieves PCOS by inhibiting granulosa cell apoptosis and oxidative stress through activating Nrf2
  Dihydrotestosterone purchased from AbMole

• 

  BMC Women's Health. 2024 Jan;25.

  PHLPP1 inhibits the growth and aerobic glycolysis activity of human ovarian granular cells through inactivating AKT pathway
  Dihydrotestosterone purchased from AbMole

• 

  Patent. CN118028239B 2024 Jul 02.

  Patent. CN118028239B
  Dihydrotestosterone purchased from AbMole

• 

  Patent. CN118028239A 2024 May 14.

  Patent. CN118028239A
  Dihydrotestosterone purchased from AbMole

• 

  Drug Chem Toxicol. 2016 Oct;39(4):392-9.

  Effects of 2,4-dichlorophenoxyacetic acid on the ventral prostate of rats during the peri-pubertal, pubertal and adult stage
  Dihydrotestosterone purchased from AbMole

• 

  J Steroid Biochem Mol Biol. 2013 Nov;138:54-62.

  Effect of androgens on different breast cancer cells co-cultured with or without breast adipose fibroblasts
  Dihydrotestosterone purchased from AbMole

Protocol (for reference only)

Cell Experiment
Cell lines	Primary pig hepatocytes
Preparation method	In steroid metabolism studies, hepatocytes were incubated in 10 ml of basal growth medium containing phenol red-free DMEM with 10% of charcoal treated FBS fortified with 10 µM (final concentration) of androstenone, 17β-estradiol or dihydrotestosterone.
Concentrations	10 µM
Incubation time	48 h

Animal Experiment
Animal models	SOD1-G93A mutant mice with the congenic C57BL/6J background
Formulation	Saline
Dosages	5 mg
Administration	silastic implant

Chemical Information

Molecular Weight	290.44
Formula	C19H30O2
CAS Number	521-18-6
Solubility (25°C)	DMSO 50 mg/mL
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Chen G, et al. PLoS One. Metabolism of androstenone, 17β-estradiol and dihydrotestosterone in primary cultured pig hepatocytes and the role of 3β-hydroxysteroid dehydrogenase in this process.

[2] Lauro FV, et al. Steroids. Design and synthesis of new dihydrotestosterone derivative with positive inotropic activity.

[3] Yoo YE, et al. PLoS One. Dihydrotestosterone ameliorates degeneration in muscle, axons and motoneurons and improves motor function in amyotrophic lateral sclerosis model mice.