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Dihydrorhodamine 123 (DHR 123) is a fluorescent probe (λex=488 nm, λem=525 nm).
In the presence of 10 μM Dihydrorhodamine 123 (DHR 123) the stimulation of the neutrophil NADPH oxidase by the addition of 50 nM phorbol 12-myristate 13-acetat (PMA) resultes in an increase in the rate of rhodamine generation. The fluorescent intensity of the cells, in the presence of 10 μM Dihydrorhodamine 123, increases with time following the addition of 50 nM PMA. In the presence of 10 μM Dihydrorhodamine 123, induced HL60 cells show a sustained increase in fluorescence following the addition of 50 nM PMA.
*The compound is unstable in solutions, freshly prepared is recommended
| Molecular Weight | 346.38 |
| Formula | C21H18N2O3 |
| CAS Number | 109244-58-8 |
| Solubility (25°C) | DMSO 90 mg/mL |
| Storage | -20°C, protect from light |
[5] N W Kooy, et al. Free Radic Biol Med. Peroxynitrite-mediated oxidation of dihydrorhodamine 123
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