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Deoxycytidine triphosphate

Cat. No. M5300

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Deoxycytidine triphosphate Structure
Synonym:

dCTP

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10mg USD 100 1-2 Weeks
50mg USD 225 1-2 Weeks
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Quality Control & Documentation
Biological Activity

Deoxycytidine triphosphate (dCTP), a nucleoside triphosphate, is a raw material in DNA synthesis. Deoxycytidine triphosphate has many applications, such as real-time PCR, cDNA synthesis, and DNA sequencing. dCTP is produced from two sources, the reduction of circulating cytidine-DP (CDP) to form dCDP, which is then phosphorylated to dCTP (the de novo pathway), and, the sequential phosphorylation of dC to dCTP (the dC salvage pathway).

Protocol (for reference only)
Cell Experiment
Cell lines HepG2 cell
Preparation method Extracts were stored at −20°C until determination of dCTP pools by a similar procedure to that described previously. Reaction mixtures (100 μl) contained 0.2μCi [3H]-dATP, 0.25 μm synthetic oligonucleotide primer, 10 mm MgCl2, 5 mm dithiothreitol, 50 mm Tris-HCl (pH 7.5) and 1 unit Sequenase 2.0 enzyme.
Concentrations --
Incubation time 24 h
Animal Experiment
Animal models
Formulation
Dosages
Administration
Chemical Information
Molecular Weight 467.16
Formula C9H16N3O13P3
CAS Number 2056-98-6
Solubility (25°C) Water 50 mg/mL
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
References

[1] Flores-Juárez CR, et al. Anal Biochem. Capacity of N4-methyl-2'-deoxycytidine 5'-triphosphate to sustain the polymerase chain reaction using various thermostable DNA polymerases.

[2] Stephen Kewn, et al. Br J Clin Pharmacol. The intracellular activation of lamivudine (3TC) and determination of 2′-deoxycytidine-5′-triphosphate (dCTP) pools in the presence and absence of various drugs in HepG2 cells

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Keywords: Deoxycytidine triphosphate, dCTP supplier, DNA/RNA Synthesis, inhibitors, activators

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