CH-223191

Biological Activity

CH-223191 is a potent and specific aryl hydrocarbon receptor (AhR) antagonist. CH-223191 inhibits TCDD-mediated nuclear translocation and DNA binding of AhR, and inhibits TCDD-induced luciferase activity with an IC50 of 0.03 μM.

*The compound is unstable in solutions, freshly prepared is recommended

Product Citations

• 

  J Neuroinflammation. 2025 Jul 10;22(1):179.

  Gut microbiota-derived indoleacetic acid attenuates neuroinflammation and neurodegeneration in glaucoma through ahr/rage pathway
  CH-223191 purchased from AbMole

• 

  Sci Rep. 2025 Dec 19; .

  Clostridium leptum attenuates allergic airway inflammation via tDC‒Treg axis activation in a murine model of gut dysbiosis-associated asthma
  CH-223191 purchased from AbMole

• 

  Patent. CN121109207A 2025 Dec 12; .

  Patent. CN121109207A
  CH-223191 purchased from AbMole

• 

  Gut Microbes. 2024 May 5;16(1):2347722.

  Microbiota-derived I3A protects the intestine against radiation injury by activating AhR/IL-10/Wnt signaling and enhancing the abundance of probiotics
  CH-223191 purchased from AbMole

• 

  Int Immunopharmacol. 2024 Feb;129:111637.

  Fraxin (7-hydroxy-6-methoxycoumarin 8-glucoside) confers protection against ionizing radiation-induced intestinal epithelial injury in vitro and in vivo
  CH-223191 purchased from AbMole

• 

  Cell Death Dis. 2023 Feb 8;14(2):92.

  Aryl hydrocarbon receptor dependent anti-inflammation and neuroprotective effects of tryptophan metabolites on retinal ischemia/reperfusion injury
  CH-223191 purchased from AbMole

• 

  J Immunol. 2023 Nov 29;ji2300090.

  The Microbial Tryptophan Metabolite Contributes to the Remission of Salmonella typhimurium Infection in Mice
  CH-223191 purchased from AbMole

• 

  Chemosphere. 2022 Nov;307(Pt 2):135962.

  Fine particulate matter induces heart defects via AHR/ROS-mediated endoplasmic reticulum stress
  CH-223191 purchased from AbMole

• 

  Advances in Clinical Medicine. 13(3), 4166-4175.

  The Regulation of Aryl Hydrocarbon Receptor (AhR) in Dry Eye
  CH-223191 purchased from AbMole

Protocol (for reference only)

Cell Experiment
Cell lines	BV-2 murine microglia cell
Preparation method	The BV-2 murine microglia cell line grown in DMEM medium with 4.5 g/L D-Glutamine, 10 % FCS and 1 % penicillin/streptomycin in standard environment. Cells were starved 24 hours before the following experiments. For protein phosphorylation detection, subcellular fractionation and immunocytochemistry assay, ITE (1 μM) was added for 4 h following with LPS (100 ng/ml) for next 4 h. For quantitative RT-PCR and western blot of inflammation cytokines, ITE (1 μM) was added for 4 hours following with LPS (100 ng/ml) for next 20 hours. CH223191 (10 μM) was pre-treated for 24 h before ITE treatment if needed.
Concentrations	10 μM
Incubation time	24 h

Animal Experiment
Animal models	Male ICR mice (6 weeks old)
Formulation	10 mg/kg in corn oil
Dosages	10 mg/kg once a day for 25 days
Administration	oral

Chemical Information

Molecular Weight	333.39
Formula	C19H19N5O
CAS Number	301326-22-7
Solubility (25°C)	DMSO ≥ 30 mg/mL
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Majid Keshavarzi, et al. Toxicol Res (Camb) . The interplay of aryl hydrocarbon receptor/WNT/CTNNB1/Notch signaling pathways regulate amyloid beta precursor mRNA/protein expression and effected the learning and memory of mice

[2] Kuo-Liang Wei, et al. Sorafenib is an antagonist of the aryl hydrocarbon receptor

[3] Jelena Kulas, et al. Aryl Hydrocarbon Receptor is Involved in the Proinflammatory Cytokine Response to Cadmium

[4] Theodoros Eleftheriadis, et al. Reoxygenation induces reactive oxygen species production and ferroptosis in renal tubular epithelial cells by activating aryl hydrocarbon receptor

[5] Lizath M Aguiniga, et al. Acyloxyacyl hydrolase regulates voiding activity

[6] Sun-Hee Kim, et al. Mol Pharmacol. Novel compound 2-methyl-2H-pyrazole-3-carboxylic acid (2-methyl-4-o-tolylazo-phenyl)-amide (CH-223191) prevents 2,3,7,8-TCDD-induced toxicity by antagonizing the aryl hydrocarbon receptor