CFDA-SE

Biological Activity

Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably, to intracellular lysine residues and other amine sources. The binding of CFDA-SE fluorescent dye to cells is stable. It is not toxic and does not modify the viability of labeled cells. CFDA-SE fluorescent dye is a suitable fluorochrome for cell labeling. CFDA-SE persists in cells 24 h after labeling, allowing the long-term detection of labeled cells.

In vivo labeling of cells with CFSE is feasible with virtually no toxic effect on the labeled or adjacent cells. It has advantages in relatively long-term migration studies as demonstrated by the recovery of T-cells in the peripheral lymphoid organs.

Another CAS#: 150206-15-8

Product Citations

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  Cell Biosci. 2026 Jan 29;16:24:00.000.

  DNA methylation-mediated silencing of MEOX1 promotes glycolysis and immune evasion in colorectal cancer cells through inhibition of GLP2R transcription
  CFDA-SE purchased from AbMole

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  ACS Nano. 2025 Jun 18;19(25):22968-22987.

  Biomimetic Ginsenoside Rb1 and Probucol Co-Assembled Nanoparticles for Targeted Atherosclerosis Therapy via Inhibition of Oxidative Stress, Inflammation, and …
  CFDA-SE purchased from AbMole

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  Adv Sci (Weinh). 2025 Mar 08; .

  Osteosarcoma Cell-Derived Migrasomes Promote Macrophage M2 Polarization to Aggravate Osteosarcoma Proliferation and Metastasis
  CFDA-SE purchased from AbMole

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  Mol Med. 2025 Aug 23;31(1):281.

  CAF-derived exosomes drive the FGF4/SHH feedback loop by encapsulating GREM1 in non-small cell lung cancer
  CFDA-SE purchased from AbMole

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  Patent. CN119818678A 2025 Apr 15; .

  Patent. CN119818678A
  CFDA-SE purchased from AbMole

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  EMBO Mol Med. 2024 Mar 6;024-00051.

  The regulatory relationship between NAMPT and PD-L1 in cancer and identification of a dual-targeting inhibitor
  CFDA-SE purchased from AbMole

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  Neural Regen Res. 2024 May;19(5):1142-1149.

  Bromocriptine protects perilesional spinal cord neurons from lipotoxicity after spinal cord injury
  CFDA-SE purchased from AbMole

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  Antibiotics (Basel). 2024 Jun 3;13(6):521.

  Antifungal Activity, Synergism with Fluconazole or Amphotericin B and Potential Mechanism of Direct Current against Candida albicans Biofilms and Persisters
  CFDA-SE purchased from AbMole

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  Cancer Lett. 2023 Jun 22;216278.

  Rapid generation of CD19 CAR-T cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia
  CFDA-SE purchased from AbMole

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  Cancer Lett. 2023 Aug 1;568:216278.

  Rapid generation of CD19 CAR-T Cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia
  CFDA-SE purchased from AbMole

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  Immunology. 2023 May 19.

  EPOR activation attenuates colitis via enhancing LC3B‐dependent apoptotic cell clearance
  CFDA-SE purchased from AbMole

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  J Nanobiotechnology. 2022 Aug 2;20(1):356.

  Lactobacillus cell envelope-coated nanoparticles for antibiotic delivery against cariogenic biofilm and dental caries
  CFDA-SE purchased from AbMole

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  Laboratory Investigation. 2022 Jul 21.

  The N6-methyladenosine writer WTAP contributes to the induction of immune tolerance post kidney transplantation by targeting regulatory T cells
  CFDA-SE purchased from AbMole

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  BioRxiv. 2022 Nov 25;517980.

  Rapid generation of CD19 CAR-T Cells by minicircle DNA enables anti-tumor activity and prevents fatal CAR-B leukemia
  CFDA-SE purchased from AbMole

Protocol (for reference only)

Cell Experiment
Cell lines	OS cells
Preparation method	To assess the ability of macrophages to phagocytose apoptotic OS cells, apoptotic OS cells were labeled with CFSE (M5117, AbMole, USA), and after cocultivation of macrophages with apoptotic OS cells for 1 h, after labeling macrophages with an anti-F4/80 flow cytometry antibody, flow cytometry assays were performed using a flow cytometer.
Concentrations	Not mentioned
Incubation time	1 h

Animal Experiment
Animal models
Formulation
Dosages
Administration

Chemical Information

Molecular Weight	557.46
Formula	C29H19NO11
CAS Number	150347-59-4
Solubility (25°C)	DMSO 80 mg/mL
Storage	4°C, protect from light

References

[1] Iñigo Terrén, et al. Methods Enzymol. CFSE dilution to study human T and NK cell proliferation in vitro

[2] Jiang X, et al. Oncoimmunology. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment.

[3] Stempin CC, et al. PLoS Negl Trop Dis. GRAIL and Otubain-1 are Related to T Cell Hyporesponsiveness during Trypanosoma cruzi Infection.

[4] Edwin D Hawkins, et al. Nat Protoc. Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data

[5] Ben J C Quah, et al. Nat Protoc. Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester

[6] Wang XQ, et al. Acta Biochim Biophys Sin (Shanghai). Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling.

[7] M E Fuller, et al. Appl Environ Microbiol. Development of a vital fluorescent staining method for monitoring bacterial transport in subsurface environments

[8] Graziano M, et al. Exp Cell Res. The fate of thymocytes labeled in vivo with CFSE.