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Blasticidin S HCl is a potent inhibitor of protein synthesis in both prokaryotic and eukaryotic cells. Blasticidin S Hydrochloride belongs to a group of cytosine amino nucleoside antibiotics. It has a wide spectrum of antimicrobial activity and acts on prokaryotic and eukaryotic organisms by inhibiting protein synthesis.
| Cell Experiment | |
|---|---|
| Cell lines | HeLa S3 cells |
| Preparation method | Twenty-four hours after transfection, the medium was changed to complete medium with blasticidin. Forty-eight hours after transfection, cells were resuspended in complete medium with blasticidin and hygromycin and split 1:4, transferring all cells from each well of the 24-well plate into wells of 6-well plates. The medium was refreshed by replacing 50% to 75% of the old medium with new one every 3 to 4 d. After 11 d, all cells in negative controls were dead and every candidate had growing colonies of selected cells. |
| Concentrations | 15 μg/ml |
| Incubation time | 3 to 4 days |
| Animal Experiment | |
|---|---|
| Animal models | |
| Formulation | |
| Dosages | |
| Administration | |
| Molecular Weight | 458.9 |
| Formula | C17H26N8O5.HCl |
| CAS Number | 3513-03-9 |
| Solubility (25°C) | Water ≥ 10 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Related Antibiotic Products |
|---|
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| Marbofloxacin
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| Linezolid
Linezolid (PNU-100766) is a synthetic antibiotic. Linezolid acts by inhibiting the initiation of bacterial protein synthesis. |
| Daptomycin
Daptomycin is the first member of a new class of bactericidal antibiotics, the cyclic lipopeptides. |
| Sulfadimethoxine
Sulfadimethoxine (trade name Di-Methox, Albon) is a sulfonamide antibiotic. |
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