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β-Galactosidase acts on the terminal β-D-galactosyl moieties of disaccharides, glycoconjugates, and polysaccharides. It is useful in mediating transglycosylation reactions such as the synthesis of galactooligosaccharides (GOS). β-Galactosidases might be used for glycobiological and biotechnological applications. β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
One unit will hydrolyze 1.0 μmole of lactose per minute at pH at 4.5 at 30 °C.
| CAS Number | 9031-11-2 |
| Solubility (25°C) | Chaotic liquid in water (mostly dissolved) Insoluble in ethanol, acetone |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
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