Angiotensin II human

Biological Activity

In vitro: Most of the known actions of Angiotensin II (Ang II) are mediated by AT1 receptors, the AT2 receptor contributes to the regulation of blood pressure and renal function. Angiotensin II raises blood pressure (BP) by a number of actions, the most important ones being vasoconstriction, sympathetic nervous stimulation, increased aldosterone biosynthesis and renal actions. Other Angiotensin II actions include induction of growth, cell migration, and mitosis of vascular smooth muscle cells, increased synthesis of collagen type I and III in fibroblasts, leading to thickening of the vascular wall and myocardium, and fibrosis. These actions are mediated by type 1 Ang II receptors (AT1). At the cellular level, responsiveness to Angiotensin II is conferred by the expression of the two classes of angiotensin receptors (AT1 and AT2). The effects of Angiotensin II to increase blood pressure are mediated by AT1 receptors.

In vivo: To distinguish the AT1 receptor population that is critical for the pathogenesis of hypertension, osmotic minipumps are implanted s.c. into each animal to infuse Angiotensin II (1,000 ng/kg/min) continuously for 4 weeks. Angiotensin II causes hypertension by activating AT1 receptors in the kidney promoting sodium reabsorption.

Product Citations

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  Mol Nutr Food Res. 2025 Jan 31.

  Chlorogenic Acid Ameliorates Chronic Unpredictable Stress-Induced Diminished Ovarian Reserve Through Ovarian Renin-Angiotensin System
  Angiotensin II human purchased from AbMole

• 

  Patent. CN119679916A 2025 Mar 25.

  Patent. CN119679916A
  Angiotensin II human purchased from AbMole

• 

  Acta Pharmacol Sin. 2024 Jan.

  Endothelial cell Orai1 is essential for endothelium-dependent contraction of mouse carotid arteries in normotensive and hypertensive mice
  Angiotensin II human purchased from AbMole

• 

  BMC Cardiovasc Disord. 2024 Feb;24(1):106.

  Sacubitril/valsartan inhibits the proliferation of vascular smooth muscle cells through notch signaling and ERK1/2 pathway
  Angiotensin II human purchased from AbMole

• 

  BioRxiv. 2023 Oct 26;564292.

  MIF Regulates M1 Macrophage Polarization via CD74/CXCR2/JNK Pathway and Mediates Aortic Dissection in Mice
  Angiotensin II human purchased from AbMole

Protocol (for reference only)

Cell Experiment
Cell lines	Human aortic (HA)-VSMCs
Preparation method	Ang II, sacubitril/valsartan were purchased from AbMole (Shanghai, China). Firstly, HA-VSMCs were seeded in serum-free medium in 6-well culture plates and cultured for 24 h after starvation. Secondly, the cells were divided into the following groups: Ang II group: Ang II(1 × 10−6 mol/L) was added directly. Ang II + Entresto group: Ang II(1 × 10−6 mol/L) was added after Entresto (1 × 10−5mol/L) worked for 1 h. Control group: this group was added without Ang II and Entresto. Each group was treated for 24 h. BMC Cardiovasc Disord. 2024 Feb 14;24(1):106.
Concentrations	1 × 10−6 mol/L
Incubation time	24 h

Animal Experiment
Animal models	(129×C57BL/6) F1 mice lacking AT1A receptors for Angiotensin II
Formulation	-
Dosages	1,000 ng/kg/min
Administration	implanted s.c.

Chemical Information

Molecular Weight	1046.18
Formula	C50H71N13O12
CAS Number	4474-91-3
Solubility (25°C)	Water 5 mg/mL DMSO
Storage	-20°C, protect from light, sealed

References

[1] Crowley SD, et al. Proc Natl Acad Sci U S A. Angiotensin II causes hypertension and cardiac hypertrophy through its receptors in the kidney.