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Andrographolide is a NF-κB inhibitor that inhibits NF-κB activation by covalently modifying the cysteine residue of P50 in endothelial cells without affecting IκBα degradation or p50/ P65 nuclear translocation. Andrographolide has antiviral effects.
| Cell Experiment | |
|---|---|
| Cell lines | MLE-12 cells |
| Preparation method | MLE-12 cells, a cell line derived from murine alveolar epithelial cells, were plated at a density of 5×103 cells per well in a 96-well plate and grown to 80% confluency in Hites media 10% fetal bovine serum. Then, cells were pretreated with 50 µM andrographolide or vehicle (0.01% DMSO) 4 h before LPS (0.5 µg/ml) stimulation. Total and nuclear proteins and mRNA were extracted from cells at specified time intervals. MTT assay was used to assess cell viability. |
| Concentrations | 50 µM |
| Incubation time | 4 h |
| Animal Experiment | |
|---|---|
| Animal models | C57BL/6 mice |
| Formulation | DMSO and Saline |
| Dosages | 2 and 4 mg/kg |
| Administration | i.p. |
| Molecular Weight | 350.45 |
| Formula | C20H30O5 |
| CAS Number | 5508-58-7 |
| Solubility (25°C) | DMSO 35.05 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Related NF-κB Products |
|---|
| QNZ
QNZ is an inhibitor of NF-κB activation with IC50 of 11 nM. QNZ (EVP4593) shows strong inhibitory effects on NF-κB transcriptional activation and TNF-α production with IC50s of 11 and 7 nM, respectively. QNZ (EVP4593) is a neuroprotective inhibitor of SOC channel. |
| Caffeic Acid Phenethyl Ester
Caffeic acid phenethyl ester is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities. |
| Cepharanthine
Cepharanthine is a biscoclaurine alkaloid inhibiting tumor necrosis factor (TNF)-α-mediated NFκB stimulation, plasma membrane lipid peroxidation and platelet aggregation and suppressing cytokine production. |
| JSH-23
Jsh-23 is a NF-κB inhibitor, inhibited NF-κB transcriptional activity in lipopolysaccharid-stimulated macrophages RAW 264.7 with IC50 of 7.1 μM. Jsh-23 inhibited the nuclear translocation of NF-κB P65 without affecting the degradation of IκBα. |
| Methylthiouracil
Methylthiouracil is an anti-thyroid agent. Methylthiouracil inhibits TNF-α and IL-6 production and NF-κB and ERK1/2 activation. |
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