All AbMole products are for research use only, cannot be used for human consumption.
Acetazolamide is a carbonic anhydrase (CA) IX inhibitor with an IC50 of 30 nM. Acetazolamide also inhibits hCA II with an IC50 of 130 nM. In vivo, Acetazolamide (40 mg/kg) significantly potentiates the inhibitory effect of MS-275 on tumorigenesis in neuroblastoma (NB) SH-SY5Y xenografts.
| Cell Experiment | |
|---|---|
| Cell lines | renal carcinoma cell lines(Caki-1, Caki-2, ACHN, and A-498) |
| Preparation method | The invasion assay is performed in a Biocoat Matrigel invasion chamber. Cells (n = 1.8 × 105) are added to the chamber and cultured for 48 h in the presence or absence of acetazolamide, which was administered twice during the 48-h period: the first dose at the beginning of the assay and the second after 24 h. After growing for 48 h, noninvasive cells are removed from the upper surface of the membrane with a cotton swab. The invasive cells on the lower surface of the membrane are fixed with 4% (vol/vol) neutral-buffered formaldehyde, stained with toluidine blue, and counted in 20 separate areas with a Leitz Diaplan microscope. |
| Concentrations | 10 μM |
| Incubation time | 48 h |
| Animal Experiment | |
|---|---|
| Animal models | Male Sprague-Dawley rats |
| Formulation | - |
| Dosages | 10, 100, 200 mg/kg |
| Administration | i.p. |
| Molecular Weight | 222.25 |
| Formula | C4H6N4O3S2 |
| CAS Number | 59-66-5 |
| Form | Solid |
| Solubility (25°C) | DMSO ≥ 25 mg/mL |
| Storage | 2-8°C, dry, protect from light, sealed |
| Related Carbonic Anhydrase Products |
|---|
| Benzthiazide
Benzthiazide is a thiazide diuretic, promoting water loss from the body. |
| Brinzolamide
Brinzolamide (Brinzolamide) is a potent and effective carbonic anhydrase-II inhibitor with an IC50 of 3.19 nM.In addition, Brinzolamide reduces intraocular aqueous humor production in the eye and may be used in studies related to glaucoma. |
| Ethoxzolamide
Ethoxzolamide is an inhibitor of the metalloenzyme carbonic anhydrase, which inhibits hCA I, hCA II, hCA IV, hCA VI and hCA VII with Ki of 25 nM, 8 nM, 93 nM, 43 nM and 0.8 nM, respectively. |
| Fluorometholone Acetate
Fluorometholone Acetate is a synthetic corticosteroid and a potent carbonic anhydrase (CA) inhibitor with IC50s of 2.18 μM for hCA-I and 17.5 μM for hCA-II. It has anti-inflammatory activity and may be used in studies related to steroid-responsive ocular inflammation. |
| Methazolamide
Methazolamide is a carbonic anhydrase inhibitor with Ki of 50 nM, 14 nM and 36 nM for hCA I, hCA II and bCA IV isoforms, respectively. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2026 AbMole BioScience. All Rights Reserved.
