All AbMole products are for research use only, cannot be used for human consumption.
For this product's availability, delivery time and price, please email inquiry@abmole.com directly or click the "Inquiry Now" button below.
6-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase.
Preparation of 6-CFDA working solution
1.Preparation of the stock solution
Dissolve 1mg 6-CFDA in 0.2172 mL DMSO to obtain 10 mM of 6-CFDA.
Note: It is recommended to store the stock solution at -20 °C -80 °C away from light and avoid repetitive freeze-thaw cycles.
2.Preparation of 6-CFDA working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of 6-CFDA working solution.
Note: Please adjust the concentration of 6-CFDA working solution according to the actual situation.
Cell staining
1.Cell preparation:
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.Add 1 mL of 6-CFDA working solution, and then incubate at room temperature for 30 minutes.
3.Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
4.Wash twice with PBS, 5 minutes each time.
5.Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
Precautions
1. It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
2. Please adjust the concentration of 6-CFDA working solution according to the actual situation.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.
| Molecular Weight | 460.39 |
| Formula | C25H16O9 |
| CAS Number | 3348-03-6 |
| Solubility (25°C) | DMSO 90 mg/mL |
| Storage | -20°C, protect from light |
| Related Fluorescent Dye Products |
|---|
| Carminic Acid (Natural dye)
Carminic acid is an anthraquinone red dye. Carminic acid is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. |
| Guanidine hydrochloride
Guanidine hydrochloride, the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant. |
| Indocyanine green
Indocyanine green(Cardiogreen) is a cyanine dye used in medical diagnostics. It is used for determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography. |
| CY7
Cy7 (Sulfo-Cyanine7) is a fluorescence labeling agent (Ex=750 nm, Em=773 nm), used to label proteins, antibodies, peptides, and oligonucleotides. |
| Fluo-4 AM
Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm). |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2026 AbMole BioScience. All Rights Reserved.
