Certificate of Analysis
| Product Name: | Ortho-iodoHoechst 33258 |
| Catalog Number: | M59231 |
| CAS Number: | 158013-41-3 |
Physical and Chemical Properties
| Formula: | C25H23IN6 |
| Molecular Weight: | 534.39 |
| Solubility: | DMSO 30 mg/mL |
| Storage: | 4°C, protect from light |
| Chemical Structure: |  |
Product Information
| Description: | Ortho-iodoHoechst 33258 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/T-rich DNA strand. Although it binds to all nucleic acids, the A/T-rich double strand DNA significantly enhances fluorescence intensity. Therefore, Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. |
| Stability and Solubility Advice: | Information concerning product stability, particularly in solution, has rarely been reported and in most cases we can only offer a general guide. We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials and used within 1 month. Avoid repeated freeze and thaw cycles. Storage conditions for some special products should refer to their storage details. |