Certificate of Analysis

 

Product Name: Cy3 TSA
Catalog Number: M66170
CAS Number: 174961-75-2

Physical and Chemical Properties
Formula: C39H47N3O8S2
Molecular Weight: 749.94
Form: Powder
Solubility: DMSO 15 mg/mL
Storage: -20°C, protect from light
Product Information
Description: Cy3 TSA is a Cy3 fluorescent dye-labelled tyramide signal amplification reagent, constituting a core component of TSA (Tyramide Signal Amplification) technology. Its mechanism of action involves horseradish peroxidase (HRP) catalysing the deposition of tyramide molecules (containing Cy3 labelling) around HRP binding sites, achieving hundreds of times amplification of the fluorescent signal. This significantly enhances detection sensitivity for low-expression targets (such as low-abundance proteins and nucleic acids), and is applicable in experiments including immunohistochemistry (IHC), immunofluorescence (IF), in situ hybridisation (ISH).
   
Stability and Solubility Advice: Information concerning product stability, particularly in solution, has rarely been reported and in most cases we can only offer a general guide.
We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials and used within 1 month. Avoid repeated freeze and thaw cycles. Storage conditions for some special products should refer to their storage details.